RUI: Pattern Formation in the Heterophyllic Aquatic Angiosperm Hippuris Vulgaris: A Study of ABA-Inducible Stomatal and Vascular Development

植物叶片发育和模式形成的许多基本方面还知之甚少。这项研究将利用一种水生植物来研究叶片中两种特定组织类型的发育--气孔和侧脉(即维管组织)。这种植物在这项研究中具有独特的优势，因为沉水植物的叶片上通常不存在这两种组织类型，但它们可以在植物生长调节剂脱落酸(ABA)诱导下分化。因此，这一系统提供了在统一的遗传背景下研究替代发展模式的机会。这项拟议的研究将结合免疫荧光显微镜和破坏微管的药物来研究微管定向和ABA诱导的气孔发育之间的关系。ABA诱导的血管发育将通过一系列实验进行研究。最后，对河马叶片发育过程中脱落酸与细胞分裂速率的关系进行了基础性研究。这项研究的结果将增加对普通沙棘气孔和维管发育的了解，包括普通植物和普通植物。Z^//

Many fundamental aspects of leaf development and pattern formation in plants are poorly understood. This research will use a species of aquatic plants to study the development of two specific tissue types - stomata and lateral veins (i.e. vascular tissue) - in leaves. This plant possesses unique advantages for this research because the two tissue types are normally absent on submerged plant leaves, but they can be induced to differentiate on plants exposed to the plant growth regulator abscisic acid (ABA). Thus, this system presents an opportunity to study alternative modes of development against a uniform genetic background. The proposed research will examine the relationship between microtubule orientation and ABA-induced stomatal development using a combination of immunofluorescence microscopy and microtubule- disrupting drugs. ABA-induced vascular development will be studied through a combination of experiments. Finally, a fundamental study will be a made of the relationship of ABA to cell division rates in developing Hippuris leaves. Results obtained from this research will increase understanding of stomatal and vascular development both in Hippuris vulgaris specifically and in plants in general. Z^//