Dissertation Research: Genetic History of Phosphoglucose Isomerase in Colias and the Coliadinae

这项研究旨在从分子水平上研究变化的动力学。它使用硫磺蝴蝶属(Colias)中的磷酸葡萄糖异构酶作为实验模型。这项研究的主要重点是比较大肠菌属和相关属的种内和种间的PGI DNA序列。然后将根据PGI上的三维结构信息、所涉及的蝴蝶的系统发育关系以及以前对Colias PGI的研究来解释序列变化。此外，这些信息将被用来解决包括Colias在内的Coliadinae内部的关系。这项拟议研究的潜在实际好处之一是，将获得与这种蛋白质的结构和功能相关的对PGI的选择性作用力的更准确的图景。

This research is designed to investigate the dynamics of change at the molecular level. It uses the enzyme phosphoglucose isomerase in the genus Colias (the sulphur butterfly) as an experimental model. The major focus of this research involves a comparison of PGI DNA sequences within and between species of Colias and related genera. Sequence changes will then be interpreted in relation to three dimensional structural information on PGI, the phylogenetic relationships of the butterflies involved, and previous studies on Colias PGI. In addition, this information will be used to address relationships within the Coliadinae, which includes Colias. One of the potential real benefits of the proposed research is that a more accurate picture of selective forces on PGI associated with the structure and function of this protein will be obtained.