Electrocatalytic Detection of Biologically Significant Compounds at Noble Metal Electrodes

这个项目是在一般地区的分析和表面 化学和电分析化学的子领域。 在这三年的持续资助任期内，教授 约翰逊和他的学生将应用脉冲电化学 极性和离子型脂肪族有机物的检测技术 具有生物学意义的化合物。 特别感兴趣的 这些研究涉及含有巯基的氨基酸和肽， 二硫化物或硫醚部分。 这些化合物传统上 不屈服于贵金属的常规电化学分析 因为它们的电化学不可逆性。 然而，这些功能部分产生瞬态阳极 金和铂电极上的信号， 电催化反应发生在形成 表面氧化物，因此可通过脉冲技术检测。 这 研究建立在约翰逊小组的成就之上， NSF基金CHE-8914700。 %%% 该研究项目的成果将扩大基础 对阳极氧化电催化反应机理认识 导致水衍生的氧转移的过程。 此外，这项研究应该引起重大的 液相色谱检测技术的进展， 传统的光度和荧光检测 低于纳摩尔水平的感兴趣的化合物需要 先验化学衍生化

This project is in the general area of analytical and surface chemistry and in the subfield of electroanalytical chemistry. During the tenure of this three-year continuing grant, Professor Johnson and his students will apply pulsed electrochemical techniques to the detection of polar and ionic aliphatic organic compounds having biological significance. Of particular interest to these studies are amino acids and peptides that contain thiol, disulfide, or thioether moieties. Such compounds traditionally do not yield to conventional electrochemical analysis at noble metal electrodes because of their electrochemical irreversibility. However, these functional moieties produce transient anodic signals at gold and platinum electrodes as a result of electrocatalytic reactions that occur during the formation of surface oxides and are thus detectable by pulsed techniques. This research builds upon accomplishments of the Johnson group under NSF grant CHE-8914700. %%% Results from this research project will expand fundamental knowledge of electrocatalytic reaction mechanisms for anodic processes that result in water-derived oxygen transfer. Additionally, this research should give rise to significant advances in detection technology for liquid chromatography wherein conventional photometric and fluorometric detection of the compounds of interest at levels less than nanomolar requires a priori chemical derivitization.