Evolution of the retrotransposons R1 and R2

R1和R2是序列特异性反转录转座因子， 插入大多数人的28S核糖体RNA基因的一部分， 节肢动物 本提案的目的是确定 这些元素的显著成功是由于它们可以自由地 从一个物种迁移到另一个物种，或者它们在 一个物种在漫长的进化过程中的谱系。 大多数实验 将集中在R1和R2的序列关系分离， 双翅目果蝇属的一个种。 第三季第2集The 3' Half of the 聚合酶链反应后， 扩增并测定其核苷酸序列。 这 序列分析将补充研究的 R1和R2在不同地理株系中的分布 几个物种使用基因组DNA印迹程序。 实验 也将进行与三种寄生蜂（属 Nasonia），其中包含多个家族的高度分歧的R1 元素 %%% 拟议的实验将提供信息， 理解移动的元件稳定性的基础 在基因组内，它们在物种之间转移的能力， 它们调节自身丰度的能力。

R1 and R2 are sequence-specific retrotransposable elements found inserted in a fraction of the 28S ribosomal RNA genes of most arthropods. The objective of this proposal is to determine whether the remarkable success of these elements is due to their freedom to move from species to species or their ability to remain active in a species lineage over long evolutionary periods. Most experiments will focus on the sequence relationships of R1 and R2 isolated from species of the dipteran genus Drosophila. The 3' half of the elements will be cloned after the polymerase chain reaction amplification and their nucleotide sequences determined. This sequence analysis will be complemented with studies of the distribution of R1 and R2 within different geographical strains of several species using genomic DNA blotting procedures. Experiments will also be conducted with three species of parasitic wasp (genus Nasonia) which contain multiple families of highly divergent R1 elements. %%% The proposed experiments will provide information that is fundamental to an understanding of the stability of mobile elements within genomes, their ability to transfer between species, and their ability to regulate their own abundance.