Bifunctional Vesicles for Enhancing Elisas

主要研究者（PI） 建议开发固相 异源酶联 免疫吸附测定（ELISA）。 的 建议的方法是使用 磷脂囊泡 （脂质体）其外表面 已经被共价修饰， 连接100至200个酶 分子以及变量，但是 特定分析物数量较少 或抗体分子。 的 潜在的信号增强， 可以通过数百人实现 附着的酶分子， 多于一个或几个，到分析物或 抗体可能会导致 的灵敏度极限， ELISA两个数量级 相对于目前的技术。

The Principal Investigators (PIs) propose to develop solid-phase heterogenous enzyme-linked immunosorbent assays (ELISAs). The proposed approach is to use phospholipid-based vesicles (liposomes) whose outer surfaces have been modified by the covalent attachment of 100 to 200 enzyme molecules as well as a variable, but smaller, number of specific analyte or antibody molecules. The potential signal enhancement that could be achieved by hundreds of attached enzyme molecules, rather than one or a few, to the analyte or antibody could result in a reduction of the limit of sensitivity of an ELISA by two orders of magnitude relative to current technology.