The Use of Recombinant DNA Technology in Undergraduate Biology Laboratories

生物学中几乎每一个分支学科目前都在使用重组DNA技术作为研究工具。随着这项重要技术的出现，生物专业和非专业本科生都要获得这项技术的实验室经验，这一点至关重要。这个项目的目标是向学生介绍基本的重组DNA实验室经验，从普通生物学课程开始，使用一套细菌重组练习。重组DNA的体验将继续在细胞生理学中进行，学生将分离出质粒DNA，进行限制性内切酶分析，连接DNA插入物，并进行转化分析。所有生物学高年级学生都必须完成一项原创研究项目。新设备将允许学生参与更多种类的项目此外，购买所需的设备将允许开发和介绍一门新的分子生物学课程。

Nearly every subdiscipline in biology is currently using recombinant DNA technology as a research tool. With the advent of this important technology, it is critical that both biology major and non-major undergraduate students gain laboratory experiences in this technology. The goal of this project is to introduce students to basic recombinant DNA laboratory experiences beginning in the General Biology Course employing a set of bacterial recombination exercises. The recombinant DNA experiences will continue in Cell Physiology where the students will isolate plasmid DNA, perform restriction enzyme analyses, ligate DNA inserts, and perform transformation assays. Completion of an original research project is required of all Biology senior students. The new equipment will allow students to participate in a wider variety of projects In addition, acquisition of the requested equipment will allow the development and presentation of a new course in molecular biology.