Equipment Acquisition to Improve an Undergraduate ImmunologyLaboratory

该项目的目的是为免疫学学生提供分析体液免疫反应的知识、程序和设备，因为它与抗原攻击和随后的抗体分析有关。学生将制备细菌全细胞和无细胞提取可溶性抗原，用于兔的后续免疫。在免疫反应研究过程中，学生将获得实际免疫学技术的实践经验，包括：1)免疫电泳；2)免疫扩散;3)免疫印迹;4)酶联免疫吸附试验。这些实验方法要求学生对实验过程和数据分析进行批判性思考。

The objective of this project is to provide immunology students with the knowledge, procedures, and equipment to analyze the humoral immune response as it relates to antigenic challenge and subsequent antibody analysis. Students will prepare bacterial whole cell and cell-free extract soluble antigens for subsequent immunization of rabbits. During the course of immune response studies students will gain hands-on experience with practical immunological techniques including: 1)immunoelectrophoresis; 2)immunodiffusion; 3) immunoblotting; and 4)enzyme-linked immunoabsorbent assays. These experimental methods will require the students to think critically about both the procedures and the analysis of their data.