Pseudo-Random Modulated Diode Laser for Time Resolved Fluorescence Spectroscopy

该提案涉及开发一种新型的， 用于时间分辨荧光的高效紧凑型传感器 生物样品的光谱学。 传感器将具有高 时间分辨率（亚纳秒），可实现精确的荧光衰减 高灵敏度的时间测量。 该传感器是基于 伪随机码调制（PRM）连续波激光雷达技术 检测来自PRM激光激发的荧光信号 由接收机系统接收，并使用交叉解调器处理， 在真实的时间内确定衰减曲线。 的时间分辨率 测量被增强到超过 数字化仪使用专有的分辨率增强 法 荧光信号的光谱信息 使用一组干涉滤光片获得。 不像在 脉冲激光荧光技术，可检测的信号不是 受背景噪声水平的限制， PRM相敏技术的特点。 在第一阶段， 我们将进行一个概念验证实验， 分析和概念系统设计。 该传感器可用于 用于荧光的体内以及远程测量 实时采样信号。

This proposal pertains to the development of a novel, cost effective, compact sensor for time resolved fluorescence spectroscopy of biological samples. The sensor will have high time resolution (sub-nanosecond) for accurate fluorescence decay time measurements with high sensitivity. The sensor is based on the pseudo random code modulated (PRM) cw lidar technique whereby the fluorescence signal from the PRM laser excitation is detected by a receiver system, and processed using cross correlators to determine the decay profile in real time. The time resolution of the measurement is enhance beyond the quantization precision of the digitizer using a proprietary resolution enhancement technique. The spectral information of the fluorescence signal is obtained using a set of interference filters. Unlike in the pulsed laser fluorescence technique, the detectable signal is not limited by the background noise level due to the inherent characteristic of the PRM phase sensitive technique. In Phase I, we will carry out a proof of concept experiment, critical system analysis, and conceptual system design. This sensor can be used for the in vivo as well as remote measurement of the fluorescence signal from samples in realtime.