Role of Agrobacterium VirB Proteins in T-DNA Transfer

本研究旨在探讨细菌感染的机制， 易感植物 根癌农杆菌的整合 T-DNA进入植物基因组导致特征性病理学。 细菌感染涉及T-DNA和某些蛋白质的转移 从细菌到植物 T-DNA和virE 2蛋白转移 是由细菌virB的产物蛋白质介导的 操纵子 计划的研究旨在：（1）表征 virB蛋白的行为，特别是可能的形成 膜相关的聚集体。 交叉连接方法将 被雇用。 virB蛋白的表达化学计量 将被审查。 单个virB蛋白在细胞凋亡中的作用 运输过程将通过个体的突变进行评估。 virB基因。 (2)运输机制本身将被审查 通过使用化学修饰或诱变修饰的virE 2 蛋白质，这可能会干扰运输机制。 %%% 植物病原菌在植物病原菌的动态中起着重要的作用， 行星生物量 更具体地说，这些病原体影响 观赏植物和农业上重要的物种。 因此，我们认为， 超越了对复杂系统组装的内在兴趣 将基因信息从细菌转移到植物， 感染过程的特征可能导致 制定有选择的干预战略， 对环境有积极影响。 计划使用的研究 生物化学和遗传学方法来表征机制 细菌感染易感植物的途径。

This is a study of the mechanism of bacterial infection of susceptible plants. Integration of the Agrobacterium tumefaciens T-DNA into the plant genome results in characteristic pathology. Bacterial infection involves transfer of T-DNA and some proteins from the bacterium to the plant. T-DNA and virE2 protein transfer is mediated by proteins which are products of the bacterium virB operon. Studies planned are aimed at: (1) characterizing the behavior of the virB proteins, particularly the possible formation of membrane-associated aggregates. Cross-linking approaches will be employed. The expression stoichiometries of the virB proteins will be examined. The roles of individual virB proteins in the transport process will be evaluated via mutations of individual virB genes. (2) The transport mechanism itself will be examined through use of chemically modified or mutagenically modified virE2 protein, which may interfere with the transport mechanism. %%% Plant pathogens play an important role in the dynamics of the planetary biomass. More specifically, these pathogens affect ornamental as well as agriculturally important species. Therefore, beyond the intrinsic interest in the assembly of a complex system to transfer genetic information from the bacterium to the plant, the characterization of the infection process may lead to development of selective intervention strategies with economic and environmentally positive consequences. The studies planned utilize biochemical and genetic approaches to characterize the mechanisms by which bacteria infect susceptible plants.