Applications of High Performance Matrix- Assisted Laser Desorption Mass Spectrometry (MALD)

9310326 Chakel这个小企业创新研究（SBIR）第二阶段项目涉及高分辨率，高质量精度的基质辅助激光解吸质谱（MALD）的开发和应用。高分辨率和高质量准确度已在第一阶段项目中的新型三重静电扇形飞行时间质谱仪上得到证明。 许多生物化学技术，如肽图谱需要准确的质量分配，以确定各种蛋白水解片段。这些片段可以在单个MALD实验中快速且明确地鉴定。第二阶段的研究将开发创新的方法，以实现生物分子的常规高质量分辨率和准确度测量。具体的应用程序说明的权力的MALD技术进行准确的生物分子分析将被开发。MALD分析的固有灵敏度、分析速度和简单性将为临床和生物研究界的直接和有价值的应用提供主要优势。 % SBIR第二阶段项目将继续第一阶段项目最初开始的进展。 SBIR计划的一个重要目标是将NSF支持的研究最终转化为技术创新和商业化。 ***

9310326 Chakel This Small Business Innovation Research (SBIR) Phase II project deals with the development and applications of high resolution, high mass-accuracy matrix-assisted laser desorption mass spectrometry (MALD). Both high resolution and high mass accuracy have been demonstrated in the Phase I project on a novel triple electrostatic sector time-of-flight mass spectrometer. Many biochemical techniques such as peptide mapping require accurate mass assignments to identify various proteolytic fragments. These fragments can be rapidly and unambiguously identified in a single MALD experiment. The Phase II research will develop innovative methods for achieving routine high mass resolution and accuracy measurements of biomolecules. Specific applications illustrating the power of the MALD technique for accurate biomolecule analysis will be developed. The inherent sensitivity, speed of analysis and simplicity of MALD analyses will provide major advantages in applications of immediate and valuable use to the clinical and biological research communities. %%% This SBIR Phase II project will continue the advances initially started in the Phase I project. An important goal of the SBIR Program is the ultimate conversion of NSF-supported research into technological innovation and commercialization. ***