U.S.-Argentina Cooperative Research: Membrane Fusion and the Biochemical Mechanism Mediating Phagocytosis

9310342斯塔尔美国-阿根廷项目奖将支持华盛顿大学的菲利普斯塔尔和阿根廷门多萨库约大学的路易斯马约尔加的合作。 该项目旨在促进吞噬作用的知识，这是一种受体介导的过程，由专门从事宿主防御的真核细胞类型进行。 该项目将探索含有新内化物质的吞噬体与适当的细胞内囊泡（内体）融合以实现内吞物质靶向的机制。 这是真核细胞的一个重要过程，对诸如单细胞生物中的食物摄取、脊椎动物免疫功能、视觉中视网膜光感受器的功能以及多细胞生物中的一般稳态等不同过程具有广泛的影响。 美国研究小组将重点研究透化细胞制剂中融合事件的重建和蛋白质因子的鉴定。 阿根廷研究小组将使用磁性乳胶球来纯化吞噬体，并确定受体在细胞内融合事件中的作用。 ***

9310342 Stahl This U.S.-Argentina Program award will support the collaboration of Philip Stahl of Washington University and Luis Mayorga of the University of Cuyo in Mendoza, Argentina. The project aims to advance knowledge of phagocytosis, a receptor-mediated process carried out by eucaryotic cell types specializing in host defense. The project will explore mechanisms whereby phagosomes, containing newly internalized materials, fuse with the appropriate intracellular vesicles (endosomes) to achieve targeting of the endocytosed materials. This is an important process for eukaryotic cells, with broad implications for such diverse processes as food uptake in unicellular organisms, vertebrate immune functions, the function of retinal photoreceptors in vision, and general homeostasis in multicellular organisms. The U.S. group will focus on the reconstitution of fusion events in permeabilized cell preparations and in identifying protein factors. The Argentine group will use magnetic latex spheres to purify phagosomes and to determine the role of receptors in intracellular fusion events. ***