Dissertation Research: Conservation Genetics of Isotria medeoloides (Orchidaceae)

9311268 Campbell北美稀有濒危兰花品种——美德洛伊索特兰的遗传变异尚不清楚。研究人员建议使用随机扩增多态性DNA （RAPD）技术，该技术允许对植物进行最小破坏性采样，以探索该物种的种群结构。为了测试稀有度和遗传变异之间的可能联系，他们将这种稀有兰花的6个种群与该属唯一的其他物种的6个种群之间的百分比可变（多态）位点进行比较。为了了解种群大小是否影响遗传变异，将对两个物种的三个大种群和三个小种群进行比较。了解该物种的种群遗传学可以更好地帮助这种联邦濒危兰花的管理者实现长期恢复目标，确定种群的相对重要性，确定最小可行种群规模，并测量总多样性随时间的变化。

9311268 Campbell Genetic variation of Isotria medeoloides a rare and endangered species of North American orchid, is unknown. The investigators propose to use Random Amplified Polymorphic DNA (RAPD), which permits minimally destructive sampling of the plants, to explore the population structure of this species. To test a possible link between rarity and genetic variability, they will contrast percent variable (polymorphic) loci between six populations of this rare orchid with that of six populations of the only other species in the genus. To see whether population size affects genetic variation, three large and three small populations for both species will be compared. %%% Knowledge of the population genetics of this species would better enable managers of this Federally Endangered orchid to meet longterm recovery goals, ascertain relative importance of the populations, define a minimum viable population size, and measure changes in total diversity over time.