DNA Amplification Fingerprinting and Molecular Diagnosis for Detecting Bacterium-like Organisms Causative of Citrus Greening Disease, US-Nepal Cooperative Research

9311802 Gresshoff描述：该项目支持尼泊尔加德满都Tirbhuvan大学植物学系的Bhaju K.Tamot博士参与田纳西大学诺克斯维尔大学植物分子遗传学系Peter H.Gresshoff博士实验室的一个研究项目。本研究利用DNA扩增指纹图谱(DAF)这一新发展起来的DNA电泳法对柑橘青枯病病原菌的DNA进行检测。将从尼泊尔受感染和健康的柑橘的叶片、花蕾和茎组织中分离出DNA。将尝试从任何细菌样有机体(BLO)中分离DNA，并产生DAF模式。感染组织和非感染组织DNA扩增产物的差异将作为检测CGD的标志。范围：这项研究带来了一位来自尼泊尔的年轻科学家，他最近获得了博士学位，在DAF技术及其在植物育种中的应用方面有一些经验，与一位在该领域发表了大量论文的美国资深科学家一起进行了研究。塔莫特博士在尼泊尔的实地工作，再加上田纳西大学与格雷肖夫博士合作开展的分析工作，应该会产生两个好处，一是扩大研究数据和信息，说明DAF作为柑橘病害早期探测器的有效性；二是对尼泊尔科学家进行更好的培训，使他能够将该技术应用于本国的其他生物目标。这是一个与发展中国家的强有力的合作项目，也是对最近一个外国博士后学位的支持。***

9311802 Gresshoff Description: This project supports the participation of Dr. Bhaju K. Tamot, of the Department of Botany, Tirbhuvan University, Kathmandu, Nepal, in a research project at the laboratory of Dr. Peter H. Gresshoff of the Department of Plant Molecular Genetics at the University of Tennessee, Knoxville. The research is to use DNA amplification fingerprinting (DAF), a newly developed DNA- electrophoretic assay, to detect the DNA of the causative agent of citrus greening disease (CGD). The DNA will be isolated separately from leaf, flower bud and stem tissue of infected and healthy citrus in Nepal. Attempts will be made to isolate DNA from any Bacterium- Like Organism (BLO), and to generate DAF patterns. The difference in DNA amplification products, one or more polymorphic bands, generated from DNA of infected and non-infected tissues with specific primer will be used as markers for detecting CGD. Scope: This research brings a young scientist from Nepal, a recent Ph.D., who has had some experience in DAF technology and its application in plant breeding, with a senior U.S. scientist who has published extensively in the field. The field work by Dr. Tamot in Nepal, combined with the analytical work at the University of Tennessee in cooperation with Dr. Gresshoff should produce two benefits, an expanded research data and information about the validity of DAF as an early detector of the citrus disease, and a better training for the Nepalese scientist that should enable him to apply the technique to other biological objectives in his country. This represents a strong cooperative project with a developing country as well as support for a recent foreign Postdoctorate. ***