Analysis and Manipulation of Cellular Metabolic Activities

9315797 San本项目研究通过遗传扰动改变E. 大肠杆菌，以减少乙酸的产生。 减少乙酸盐形成旨在减少产物抑制，从而增加重组蛋白生产和细胞生长。 乙酸盐的减少是由于引入乙酰乳酸芽孢杆菌合成基因（ALS）引起的，该基因引起乙偶姻形成的增加。 这项研究将成为利用代谢工程提高产品产量的一个例子。 添加ALS基因对各种反应通量，特别是与丙酮酸分支点相关的反应通量再分配的影响将是本研究的重点。 此外，这些通量变化的建模和分析将通过调整已建立的路径分析方法来进行。 ***

9315797 San This project is on research to redirect, by a genetic perturbation, the cellular carbon fluxes in E. coli in order to reduce acetate production. Decreased acetate formation is intended to reduce product inhibition and thereby increase both recombinant protein production and cell growth. The decrease in acetate is caused by the introduction of a Bacillus acetolactate synthesis gene (ALS) which causes an increase in the formation of acetoin. This research will be an example of the use of metabolic engineering to increase product yields. The effect of the added ALS gene on the redistribution of various reaction fluxes, especially those associated with the pyruvate branch point, will be the focal point of this research. In addition, the modeling and analysis of these flux changes will be performed by adapting established pathway analysis methodologies. ***