Enchancement and Upgrade of the DNA Sequencing Facility: Upgrade of Automated Sequencer (ABI 37 3A)/Electrophoresis Unit

我们建议购买一些设备和软件，通过升级我们的应用生物系统373A电泳装置（=自动测序仪）及其相关设备，增强我们DNA测序核心设施的能力和服务，并为用户提供增强的数据分析工作站访问权限。硬件包括一个五过滤轮（允许使用T7 DNA聚合酶进行测序），一个线性升级（允许使用几个可变的易读凝胶板），垂直琼脂糖凝胶板（便于分析2-3 kb片段和随机扩增的多态DNA），以及计算机设备（提高分析速度并适应多个用户）。该软件包括便于序列比对的材料，提高片段评分的速度，并创建一致的序列。这些增强将服务于不同学科的多样性研究项目，从系统发育重建（来自极端环境的蓝藻，主要甲壳类谱系）到基因表达检查（衣藻的鞭毛再生，肌肉组织的适应变化），再到确定自然种群的生殖模式（繁殖鱼类的交配）。此次升级将扩大该设施可以提供的应用范围，并允许更快速的样品分析，从而使来自多个学科的用户能够轻松扩展他们的工作范围并提高他们的生产力。

We are proposing to purchase a number of items of equipment and software that will enhance the capabilities and services of our DNA Sequencing Core Facility by upgrading our Applied Biosystems 373A Electrophoresis Unit (= automated sequencer) and its associated equipment and providing the users with enhanced access to data-analysis work stations. The hardware includes a five-filter wheel (to permit sequencing with T7 DNA polymerase), a liner upgrade (to permit the option of using several variable well-to-read gel plates), vertical agarose gel plates (to facilitate analysis of 2-3 kb-fragments and random amplified polymorphic DNA), and computer equipment (to increase analytical speed and accommodate multiple users). The software includes material to facilitate sequence alignment, increase the speed of scoring fragments, and create consensus sequences. The enhancements will serve a diversity of research projects in different disciplines, from phylogenetic reconstructions (cyanobacteria from extreme environments, the major crustacean lineages) to examinations of gene expression (flagellar regeneration in Chlamydomonas, acclimatory change in muscle tissue) to the determination of reproductive patterns in natural populations (mating in livebearing fishes). The upgrade will expand the range of applications that the facility can offer and permit more rapid sample analysis, thereby enabling users from several disciplines to expand readily the scope of their work and to increase their productivity.