Pseudo-Random Modulated Diode Laser for the Time Resolved Fluorescence Spectroscopy

本提案涉及到一个原型亚纳秒分辨率光学传感器的开发利用伪随机调制（PRM）相关技术的时间分辨荧光或光子迁移测量。第一阶段的概念验证实验和伴随的模拟研究结果清楚地证明了该技术在时间分辨测量方面的强大能力。在荧光和光子迁移实验中验证了时间分辨率增强技术的有效性。此外，本文还提出了一种新的校正方法，即利用测量到的泵源波形作为参考编码，对信号下垂引起的波形变形进行校正。结合强大的prm相关方法与特设参考代码和分辨率增强技术，一种简单，成本有效的亚纳秒时间分辨率的荧光传感器现在被概念化。在第二阶段，将开发一个分辨率为50 psec的光子迁移测量原型传感器，并对该系统进行测试，用于测量模拟组织样品的时间分辨反射率，以检索样品的相关参数。此外，还将研究开发可行的prm相关荧光传感器蓝色激光源。

This proposal pertains to the development of a prototype sub-nanosecond resolution optical sensor utilizing Pseudo-Random Modulation (PRM) Correlation technique for time resolved fluorescence or photon migration measurements. The results of the Phase I proof-of-concept experiment and accompanying simulation studies clearly demonstrated the powerful capabilities of the technique for time resolved measurements. The validity of the Time Resolution Enhancement Technique was verified in both fluorescence and photon migration experiments. In addition, a new method for correcting waveform deformation due to the signal droop, is developed by using an ad-hoc reference code which is simply the measured pump source waveform. Combining the powerful PRM-Correlation method with the ad-hoc reference code and the Resolution Enhancement Technique, a simple, cost effective fluorosensor with sub-nanosecond time resolution is now conceptualized. In Phase II, a prototype sensor for photon migration measurement with 50 psec resolution will be developed, and the system will be tested for the measurement of time resolved reflectance from a simulated tissue sample to retrieve the relevant parameters of the sample. In addition, research for development of viable blue laser sources for PRM-Correlation fluorosensor will also be performed.