Investigative Instructional Improvements in Molecular Biology and Organellar DNA Analysis

这个项目的目的是促进学生在研究分子生物学方面的培训和经验。具体地说，所要求的设备包括一台高速离心机和转子、热循环仪、电穿孔器、核苷酸测序和琼脂糖凝胶电泳仪以及相关附件，将支持一门新的研究密集型高级调查分子生物学课程，在该课程中，学生将描述藻类线粒体和叶绿体基因组结构和基因结构。拟议的设备将用于提高用于核酸分析的策略的复杂性，包括纯化的细胞器DNA的分离、DNA文库的构建、基因组图谱和选定基因的核苷酸序列分析。DNA结构和功能的基本原理和基本技术在现有的课程中介绍。调查分子生物学将提供以下机会：(1)提供DNA组织和功能的高级研究；(2)作为学生参与调查性研究的途径；以及(3)对于生物学专业，培训和关注那些预期进入分子生物学相关荣誉研究项目的学生。

The aim of this project is to advance student training and experience in investigative molecular biology. Specifically, the requested equipment, consisting of a high speed centrifuge and rotors, thermocycler, electroporator, nucleotide sequencing and agarose gel electrophoresis equipment and related accessories, will support a new research-intensive advanced investigative molecular biology course in which students will characterize algal mitochondrial and chloroplast genome architecture and gene structure. The proposed equipment will be used to increase the sophistication of strategies used for nucleic acid analysis including the isolation of purified organellar DNA, DNA library construction, genome mapping, and nucleotide sequence analysis of selected genes. Fundamental principles of DNA structure and function and basic techniques are presented in existing intorductory courses. Investigative Molecular Biology will offer opportunities that (1) provide for advanced study of DNA organization and function; (2) serve as an avenue for student participation in investigative research; and (3) for biology majors, function to train and focus those students who anticipate entering into molecular biology related honors research projects.