Molecular Cytoskeletal Microscopy Probes and Biological Wires

9360219 Powell Novel探针被提议用于通过电子显微镜研究细胞骨架结构和过程。 这些将包括直径为0.8 nm - 2.4 nm的金、铂和钯的大簇络合物，其通过结合到它们的配体球中的单个反应性官能团与鬼笔环肽、细胞松弛素B、秋水仙素和紫杉醇共价连接。 所提出的缀合物将穿透20-30 μ或更多进入细胞切片，并实现比胶体金=抗体缀合物显著更高的分辨率。 它们将是可微注射的，与活细胞相容，并将用于将电子显微镜的使用扩展到研究生物体，如原核细菌，其中抗体探针是不可行的。 包括金簇和荧光部分的组合标记将用于制备具有相同分子的另外的探针，从而使来自单个标记实验的荧光共聚焦显微镜和电子显微镜数据相关。 将尝试通过体外组装金标记的细胞骨架成分，然后通过银增强来制备分子导线。 一类新的囊骨结构和过程的探针，与活细胞兼容，将允许电子显微镜具有比抗体探针更高的分辨率。 标记细胞骨架组分的体外组装将用于制备新型导电分子线，并可能应用于其他分子器件、催化剂、新材料和新型分子检测系统的制造。 ***

9360219 Powell Novel probes are proposed for the study of cytoskeletal structures and processes by electron microscopy. These will comprise large cluster complexes of gold, platinum and palladium 0.8 nm - 2.4 nm in diameter covalently lined to phalloidin, cytochalasin B, colchicin and taxol via a single reactive functional group incorporated into their ligand spheres. The proposed conjugates will penetrate 20-30 u or more into cell sections and achieve significantly higher resolution than colloidal gold=antibody conjugates. They will be microinjectable and compatible with living cells, and will be used to extend the use of electron microscopy to the study of organisms such as procaryotic bacteria, in which antibody probes are not feasible. Combined labels including both a gold cluster and a fluorescent moiety will be used to prepare additional probes with the same molecules, enabling correlation of fluorescence confocal microscopy and electron microscopy data from a single labeling experiment. The preparation of molecular wires will be attempted by the in vitro assembly of gold-labeled cytoskeletal components, followed by silver enhancement. %%% A new class of probes for cystoskeletal structures and processes, compatible with living cells, will permit electron microscopy with higher resolution than antibody probes. In vitro assembly of labeled cytoskeletal components will be used to prepare novel conductive molecular wires, and may be applied to the fabrication of other molecular devices, catalysts, new materials and novel molecular detection systems. ***