Specific Phage Particles for Bio-Sorbtion of Heavy Metals in Waste Waters

9361633将使用噬菌体展示技术开发噬菌体颗粒，其特异性结合重金属（汞和铬）。通过将简并核苷酸克隆到编码噬菌体M13外壳蛋白的基因中来产生随机噬菌体文库。 所得到的噬菌体将有多达1013个随机肽中的一个暴露在其表面上，多达2700个拷贝。 将筛选所得噬菌体颗粒以选择特异性结合重金属的那些。 选定的噬菌体颗粒将用于从各种废水中去除金属，包括工业过程、核设施、土壤洗涤以及废物的不当储存或处置所产生的废水。 预计使用噬菌体结合来预处理混合有机（有机物+金属）废物以去除金属将允许成功地生物处理美国许多迄今为止无法处理的废物场地。 这项技术的成功示范将为今后开发补救更难处理的废物，如混合低放射性废物（有机物+放射性核素）提供先例。 这项研究的完成将有助于开发一种商业上可行的生物处理系统，用于处理含有有机污染物和重金属混合物的危险废物。 ***

9361633 Steffan Bacteriophage particles will be developed using phage display technology that specifically bind heavy metals (mercury and chromium). Random phage libraries will be generated by cloning degenerate nucleotides into genes encoding coat proteins of the bacteriophage M13. The resulting phage will have one of as many as 1013 random peptides exposed on their surface in up to 2700 copies. The resulting phage particles will be screened to select those that specifically bind heavy metals. Selected phage particles will be used to remove metals from a variety of wastewaters including those generated by industrial processes, nuclear facilities, soil washing, and improper storage or disposal of wastes. %%% It is expected that using phage binding to pre-treat mixed organic (organics + metals) wastes to remove metals will allow for the successful biotreatment of many of the nation's heretofore untreatable waste sites. The successful demonstration of this technology will provide a precedent for the future developments to remediate even more difficult wastes such as mixed low-level radioactive wastes (organics + radionuclides). Completion of this research will allow the development of a commercially-viable biological treatment system for hazardous wastes containing mixtures of organic contaminants and heavy metals. ***