Viral Amplification of Recombinant RNA In Transgenic Plants

9402103植物与病毒的相互作用为生产复杂分子提供了独特的机会。烟草花叶病毒(TMV)是烟草花叶病毒属的典型成员，是生物学各方面研究最深入的病毒之一。利用现有的构建重组RNA病毒和转基因宿主的技术，我们建议使用病毒RNA扩增来合成植物中的特定产物。在第二阶段，我们将确定必要的和最优的上体序列配置，以实现在整个植物中实现受控和生物包含的高水平基因表达的目标。%植物中最高表达的基因是在植物RNA病毒基因组中发现的。为了开发植物病毒作为基因载体，我们将设计保持产生感染性重组体的能力的构建体，因此，它们作为基因载体的使用将不再受到限制。因此，复杂分子的商业生产将得到极大的优化。***

9402103 Abstract Turpen The interaction of plants and their viruses present unique opportunities for the production of complex molecules. Tobacco Mosaic Virus (TMV) is the type member of the tobamovirus group and is one of the best understood viruses in all aspects of their biology. Using existing technology for the construction of recombinant RNA viruses and transgenic hosts, we propose to use viral RNA amplification for the synthesis of defined products in plants. In Phase II, we will determine the necessary and optimal configuration of episomal sequences to achieve the goal of controlled and biologically contained, high level gene expression in whole plants. %%% The most highly expressed genes in plants are found encoded in plant RNA viral genomes. In order to develop a plant virus as a gene vector, we will design constructs which retain the ability to generate infectious recombinants and, as such, will no longer be limited in their use as gene vectors. Thus, the production of complex molecules on a commercial basis will be greatly optimized. ***