A Study of Wnt/Wingless Signalling

9418793齐格弗里德该提案的目标是了解调节果蝇黑腹果蝇胚胎发育的细胞-细胞通讯机制。 无翅蛋白是一种在胚胎细胞亚群中表达的蛋白质，它可以向不表达无翅蛋白的邻近细胞发出信号，并调节它们的命运。这种细胞信号传导的机制尚不清楚，但我们已经确定了一些参与信号传导途径的其他分子。 Zeste白色3激酶是该途径的一个组成部分，它能够通过向其他蛋白质中添加磷酸盐来修饰它们，并可能改变它们的活性。 我们建议鉴定Zeste白色3激酶修饰的蛋白质，并确定Zeste白色3激酶活性本身是如何调节的。 对Zeste白色3的研究将使我们更好地理解无翼信号。这与理解脊椎动物的生长和发育有关，因为无翼与小鼠中发现的正常发育所需的蛋白质相似。 此外，无翅信号通路可能在脊椎动物中是保守的，因为诸如Zeste白色3的组分也具有脊椎动物对应物。 ***

9418793 Siegfried The goal of this proposal is to understand the mechanism of cell- cell communication that regulates the development of the fruit fly Drosophila melanogaster embryo. Wingless is a protein that is expressed in a subset of cells in the embryo that can signal to neighboring cells that don't express the Wingless protein and regulate their fate. The mechanism of this cell signalling is unknown but we have identified a number of other molecules that are involved in the signalling pathway. Zeste white 3 kinase, which has the ability to modify other proteins by adding phosphate to them, and potentially alter their activity, is one component of this pathway. We propose to identify the proteins which Zeste white 3 kinase does modify and to determine how Zeste white 3 kinase activity is itself modulated. The investigation of Zeste white 3 will lead to a greater understanding of the Wingless signalling. This is relevant to the understanding of vertebrate growth and development since Wingless is similar to proteins found in mice that are required for normal development. In addition, the Wingless signalling pathway is likely to be conserved in vertebrates since components such as Zeste white 3 also have vertebrate counterparts. ***