Analysis of Oxidative DNA Repair Enzymes in Damaged Rat Kidney (Biology)

本研究包括两种酶的研究，氧化酶核酸内切酶和甲脒嘧啶/8-氧鸟嘌呤-DNA糖基化酶，它们启动DNA中氧化碱基损伤的修复。这两种酶的水平将通过生化分析在遭受生理氧化应激的大鼠肾脏组织提取物中进行量化，并与来自同一动物的未受损肾脏组织以及未经处理的对照进行比较。这些研究结果将有助于更好地理解DNA氧化损伤诱导DNA修复活性，并为进一步研究自由基介导的动物组织损伤的基本机制提供基础。互动活动包括：教授修订后的研究生水平药理学课程，重点是分子生物学原理和技术；组织小组讨论，重点讨论男性和女性从事科学研究的差异；并为本科生和研究生以及感兴趣的教师提供重组DNA技术短期课程。

This research includes a study of two enzymes, the redoxyendonuclease and formamidopyrimidine/8-oxyguanine-DNA glycosylase, which initiate the repair of oxidative base damages in the DNA. The levels of both enzymes will be quantified by biochemical assays in extracts of rat kidney tissue subjected to physiological oxidant stress as compared to the undamaged kidney tissue from the same animal as well as untreated controls. The results of these studies will lead to a better understanding of the induction of DNA repair activities in response to oxidative DNA damage and provide a basis for future studies on the fundamental mechanisms underlying free radical-mediated damage in animal tissues. Interactive activities include: teaching a revised graduate level pharmacology course with emphasis on molecular biology principles and techniques; organizing a panel discussion focusing on the differences in which men and women conduct science; and offering a recombinant DNA technology short course to undergraduate and graduate students as well as interested faculty.