Instrumentation for an Advanced Undergraduate Laboratory in Biochemistry and Molecular Biology

9451005蔡高等生物化学与分子生物学专业本科生强化实验课程《高等生物化学与分子生物学实验室（化学5362）》是新开设的生物化学与分子生物学理学学士专业必修的高级课程。该实验室的一个主要特点是学生自己完成所有的程序，而不涉及任何“示范”练习。所使用的练习将包括学生以后在该领域的职业生涯中可能遇到的最先进的技术。这些技术包括计算机辅助DNA序列分析、聚合酶链反应（PCR）、DNA电穿孔、同源重组、体外诱变、报告基因融合、大肠杆菌真核蛋白的生产、生物分子的自动化低压液相色谱和高压液相色谱（HPLC）。这些技术的“实践”经验会让学生在未来的努力中具有竞争优势。

9451005 Tsai An intensive laboratory course for undergraduates in advanced techniques of biochemistry and molecular biology -Advanced Biochemistry and Molecular Biology Laboratory (Chem 5362)-is a senior level course, required by the new Bachelor of Science program in Biochemistry and Molecular Biology. A key feature of the lab is that the students perform all procedures themselves without involving any "demonstration" exercises. Exercises used will include state-of-the-art techniques that the students are likely to encounter later in their careers in this field. These include computer-assisted DNA sequence analysis, polymerase chain reaction (PCR), DNA electroporation, homologous recombination, in vitro mutagenesis, reporter gene fusions, production of eukaryotic proteins in E. coli, automated low pressure liquid chromatography, and high pressure liquid chromatography (HPLC) of biological molecules. A "hands-on" experience with techniques such as these gives students a competitive edge in their future endeavors.