NSF Young Investigator: Controlled Radical Delivery Systems for Understanding DNA Structure

这个NSF青年研究者奖在无机，生物无机和有机 该计划为Charles G.博士的生物无机化学研究提供支持。赖尔登 堪萨斯州立大学化学系 水溶性有机金属配合物 将用作碳自由基的光解源，碳自由基将与核酸反应。 已发现一类钴络合物可切割单链和双链DNA。 这些配合物的有机衍生物将被制备以提供不同种类的烷基自由基，并且将确定这些自由基对DNA的攻击的特异性。 这些烷基基团中的一些的空间体积可能导致序列特异性，因为 识别DNA的三级结构。 此外，以这种方式产生的甲基自由基将是 用作足迹探针以鉴定金属蛋白-DNA结合区域。

This NSF Young Investigator Award in the Inorganic, Bioinorganic, and Organometallic Program provides support for research in bioinorganic chemistry by Dr. Charles G. Riordan of the Chemistry Department, Kansas State University. Water-soluble organometallic complexes will be used as photolytic sources for carbon radicals which will be reacted with nucleic acids. A class of cobalt complexes has been found to cleave both single- and double-stranded DNA. Organometallic derivatives of these complexes will be prepared to deliver different kinds of alkyl radicals and the specificity of the attack of these radicals on DNA will be determined. The steric bulk of some of these alkyl radicals may result in sequence specificity because of recognition of DNA's tertiary structure. Also, methyl radicals produced in this manner will be used as footprinting probes to identify metalloprotein-DNA binding regions.