CAREER: Strategies for Metabolic Engineering of Bacteria: Novel Synthesis of Biodegradable Polymers

9502495 Keasling这个项目正在研究开发一种方法，通过这种方法可以改变细菌代谢的方向，以合成所有必要的前体，或从廉价的碳源生产所需的复杂化合物，并以能够实现特定成分的方式控制新陈代谢。这项研究的总体目标是：(1)将来自不同生物体的多条代谢途径引入单个细菌：(2)确定如何在足以满足功能复合体的化学计量水平上平衡酶亚单位的生产；(3)开发一个数学模型来预测如何平衡所需产品所需的多个通量；以及(4)在所需水平上协调表达编码该途径的酶的基因。除了这项研究活动外，调查员还将通过开展相关的实验室实验来加强教育。***

9502495 Keasling This project is on research to develop a methodology whereby bacterial metabolism can be redirected to synthesize all precursors necessary or the production of a desired, complex compound from an inexpensive carbon source, and to control metabolism in such a way that a defined composition can be achieved. The general aims of the research are: (1) to introduce multiple metabolic pathways from various organisms into a single bacterium: (2) to determine how to balance production of enzyme subunits at stoichiometric levels sufficient for a functioning complex: (3) to develop a mathematical model to predict how to balance more than one flux necessary for a desired product: and (4) to coordinately express the genes encoding the enzymes for the pathway at the desired level. In addition to this research activity, the investigator will enhance education by developing relevant laboratory experiments. ***