Soluble Methane Monooxygenase Expression and Co-oxidation Activity under Nitrogen-Limited Conditions (Collaborative Research)

本项目旨在通过提高甲烷营养细菌中可溶性甲烷单加氧酶（sMMO）的表达和活性来改善卤代化合物的生物修复。该策略是在不同的铜、氮和氧环境中培养野生型和突变型菌株，同时监测sMMO和氮酶的表达和活性。将对选定菌株的各种环境污染物的共氧化速率进行量化，并注意氮限制的影响。这些污染物包括卤代乙烷、卤代甲烷和某些杀虫剂。最后，通过一个模型将表达和活动模式与环境条件联系起来，该模型将用于开发反应堆设计。＊＊＊

9504383 Taylor This project is on research to improve the bioremediation of halogenated compounds by increasing the expression and activity of the soluble methane monooxygenase (sMMO) enzyme in methanotrophic bacteria. The strategy is to culture wild-type and mutant strains of bacteria in various copper, nitrogen, and oxygen environments, while monitoring sMMO and nitrogenase expression and activity. The co-oxidation rates of a variety of environmental pollutants will be quantified for selected strains, with attention to the influence of nitrogen limitation. These pollutants include halogenated ethanes, halomethanes and selected pesticides. Finally, the expression and activity patterns will be related to the environmental conditions by a model which will be used to develop reactor designs. ***