International Postdoctoral Fellows Program: Antigene Strategies of Protein DNA Interfaces

9505345佩林国际初级研究员和博士后研究员计划使美国科学家和工程师能够在国外进行为期3至24个月的研究。该项目的奖项提供了联合研究的机会，并提供了使用国外独特或互补的设施、专业知识和实验条件的机会。这一奖项将支持大卫·M·佩林博士为期12个月的博士后研究访问，与法国国家历史博物馆的克劳德·海伦教授合作。反义技术几乎涵盖了所有旨在理性产生分子的研究，这些分子可以与DNA和/或RNA结合来阻止蛋白质的产生，抑制细胞的生长和分裂，甚至杀死细胞。本项目的目标是通过探索1)医学上重要的DNA损伤功能与寡核苷酸的合成偶联，2)更有弹性的核苷酸的合成和性质，3)通过合成嵌入物的偶联来增加相互作用的稳定性，并通过分光光度技术表征反义相互作用，以及4)产生其他序列，其抑制特性将在细菌和哺乳动物细胞系统中被检测。***

9505345 Perrin The International Junior Investigator and Postdoctoral Fellows Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. David M. Perrin to work with Professor Claude Helene of the Museum National d'Histoire Naturelle in France. Antisense technology encompasses virtually all research directed toward the rational generation of molecules which can associate with DNA and/or RNA to block protein production, suppress cell growth and division, or even kill the cell. The goal of this project is to characterize antisense interaction by exploring 1) the synthetic coupling of medicinally important DNA-damaging functionalities to the oligonucleotide, 2) the synthesis and properties of more resilient nucleotides, 3) the synthetic coupling of intercalators to increase the stability of the interaction and to characterize the antisense association by spectrophotometric techniques and 4)the generation of other sequences whose inhibitory properties will be examined in- vivo in bacterial and mammalian cell systems. ***