Acquisition of an Electronic Particle Counter

确定了对电子粒子计数器的迫切需要，并要求提供资金购买Coulter公司生产的Multisizer lle电子粒子计数器。本研究将利用粒子计数器（1）研究蚊幼虫的摄食生态，以及摄食率和粒子选择与幼虫发育部位营养状态的关系;（2）通过对花粉产生、传粉者移除的花粉量和授粉成功率等性状进行量化，研究自然选择、群体遗传学和植物生殖生态学，（3）确定蚊子幼虫对不同颗粒类型的选择以及摄食生态学中的行为变化（导致对可摄入毒素的抗性）对颗粒选择的影响。 喂养率，并最终对库蚊的抗性与敏感品系的生活史;（4）测量蚊子发育部位的颗粒大小分布和丰度;（5）定量和大小封闭的杆状病毒。除了满足主要和次要用户的需求外，粒子计数器还将促进与加州大学滨江（UCR）和其他机构的同事的合作，满足在我们实验室工作的博士后科学家和研究生的需求，并加强UCR本科生和研究生的教育。粒子计数器将由研究人员使用，将不同的方法应用于各种粒度。因此，请求提供资金再购买四个孔径。还需要经费购买为Multisizer lle设计的软件、一台独立的IBM兼容计算机和一台打印机。与价格低廉的电子粒子计数器相比，Multisizer lle系统的优点包括操作简单，粒子动态范围更大， 尺寸、显著增强用户安全性的功能、灵活且用户友好的软件、减少来自外部源的电子干扰的功能。和上级用户支持服务。

A critical need for an electronic particle counter is identified and funds are requested to purchase a Multisizer lle electronic particle counter made by Coulter Corporation. The particle counter will be used (1 ) to study the feeding ecology of larval mosquitoes and the relationship of feeding rates and particle selection to trophic state of larval developmental sites; (2) to study natural selection, population genetics, and plant reproductive ecology by quantifying traits such as pollen production, the amount of pollen removed by pollinators and pollination success, and by examining variation and environmental influences on the male component of plant reproductive success; (3) to determine the selection of different particle types by mosquito larvae and the impact of behavioral changes in feeding ecology (which cause resistance to ingestible toxins) on particle selection. feeding rates and, eventually, on the life histories of resistant vs susceptible strains of Culex mosquitoes; (4) to measure particle size distributions and abundance in mosquito developmental sites; and (5) to quantify and size occluded bacculoviruses. In addition to fulfilling the needs of the major and minor users, a particle counter will foster collaborations with colleagues at University of California - Riverside (UCR) and other institutions, meet the needs of postdoctoral scientists and graduate students working in our laboratories, and enhance the education of undergraduate and graduate students at UCR. The particle counter will be used by investigators applying diverse methodologies to a wide range of particle size. Therefore, funds are requested to purchase four additional aperatures. Funds are also requested to purchase software designed for the Multisizer lle, a free-standing IBM-compatible computer and a printer. As compared to comparably priced electronic particle counters, the advantages of the Multisizer lle system include ease of operation, a greater dynamic range of particle size, features that significantly enhance user safety, flexible and user-friendly software, features that reduce electronic interference from outside sources. and superior user-support services.