Characterization of a Novel Group I Intron in Bacillus Bacteriophage FS7

;​R o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o4 @ 9:；= ?@ A B F Microsoft Word 6.0文档MSWordDoc6;​9520503 Wilson PRIVATE申请职业发展奖励，将学生从锡耶纳学院的休假延长一个学期；在返回原院校后，提供额外一学期的教学空闲时间；并提供暑期工资支持。她希望在纽约州立大学奥尔巴尼分校大卫·舒布博士的实验室休假期间继续开展一项有前途的研究。在建议的资助期内，她还计划就该项目向美国国家科学基金会本科院校研究项目申请研究经费，以便继续与本科生一起进行研究。对内含子的系统搜索将用于探索原核生物基因中I族内含子之间的进化关系。最近在枯草芽孢杆菌噬菌体胸苷合酶（TS）基因中发现了一个自剪接内含子，B22 （thy）与先前鉴定的T4噬菌体胸苷合酶（td） I组内含子以及其他已知的T4内含子（nrdB和sunY）具有许多相同的特性。一些与~22结构相同的革兰氏（+）细菌候选噬菌体将通过PCR方法寻找其TS基因内的内含子。每个新发现的内含子将被克隆到体外转录载体中，分析其从初级转录本中自我剪接的能力，并对其进行测序以推断其折叠结构，并确定其与已知的I族内含子的相关性。虽然绝大多数真核生物的基因都含有内含子，但直到在噬菌体T4的胸苷酸合成酶基因中惊人地发现了一个自剪接内含子之前，没有一个被确定存在于真细菌或它们的病毒中。T4中的其他人也很快被发现了。在感染的宿主细胞中，T4中所有含内含子的基因编码参与脱氧核糖核苷酸合成的蛋白质。在职业发展奖中，将对原核基因中的内含子进行系统的搜索。每个新发现的内含子将被分析其自剪接能力，并确定其结构和与其他I族内含子的关系。*** ;@ ....（）（）) ()() ()() Z：凤凰W ho；如果你是我，我是你，我是你，我是你，我是你，我是你Oh +' 0 $ H l D H R:\WWUSER\TEMPLATE\NORMAL。DOT marcia steinberg marcia steinberg @ t @ @ @ @ @ j6 Microsoft Word 6.0 3e = e " " " " " " " L L L L L L L L L LC x | | | | | | | # f W T 4 " | | | | | | " " | x | | | | " | " | 6 " " " " | | 5 |威尔逊私人：这是一份关于职业发展的提案

; R o o t E n t r y F o/ @ C o m p O b j b W o r d D o c u m e n t O b j e c t P o o l o/ @ o/ @ 4 @ 9 : ; = ? @ A B F Microsoft Word 6.0 Document MSWordDoc Word.Document.6 ; 9520503 Wilson PRIVATE This proposal is for a Career Advancement Award to extend the PIs sabbatical leave from Siena College for one semester; to provide release time from teaching for an additional semester upon return to her home institution; and to provide summer salary support. She wishes to continue a promising line of research initiated during a sabbatical in Dr. David Shub's laboratory at the State University of New York at Albany. During the proposed period of support she also plans to write and submit a research grant on this project to the NSF Research in Undergraduate Institutions program so that she can continue the investigations in with undergraduates. A systematic search for introns will be undertaken to explore the evolutionary relationships between Group I introns in prokaryotic genes. A recently discovered self splicing intron within the thymidylate synthase (TS) gene of B. subtilis bacteriophage ,B22 (thy) shares a number of properties with the previously identified thymidylate synthase (td) Group I intron of bacteriophage T4, as well as with the other known T4 introns (nrdB and sunY). A number of candidate phages of gram(+) bacteria, identical in structure to ~22, will be explored by PCR for introns within their TS genes. Each new intron discovered will be cloned into an in vitro transcription vector to analyze its ability to self splice from primary transcripts, and sequenced to deduce its folded structure, and ascertain its relatedness to the known Group I introns. %%% Although the vast majority of eukaryotic genes contain introns, none has been identified as residing in eubacteria or their viruses until the surprising discovery of a self splicing intron in the thymidylate synthase gene of bacteriophage T4. Others in T4 were quickly discovered as well. All of the intron containing genes in T4 encode proteins involved in the synthesis of deoxyribonucleotides in infected host cells. In the Career Advancement Award, a systematic search for introns in prokaryotic genes will be undertaken. each new intron identified will be analyzed for its ability to self-splice, and its structures and relatedness to other group I introns will be determined. *** ; @ ....()()))()() Z : phoenix W EH o ; S u m m a r y I n f o r m a t i o n ( 8 Oh +' 0 $ H l D h R:\WWUSER\TEMPLATE\NORMAL.DOT marcia steinberg marcia steinberg @ t @ @ @ @ @ J 6 Microsoft Word 6.0 3 e = e " " " " " " " L L L L L d n L C x | | | | | | | # f W T 4 " | | | | | | " " | x | | | | " | " | 6 " " " " | | 5 | 9520503 Wilson PRIVATE This proposal is for a Career Advan