Reactor Design and Scale-up of Plant Root Cultures

9522033 Curtis该提案是关于研究开发反应器设计和操作原理，用于大规模培养根培养物，以生产生物活性化学品，如药物，农药和香料。 初步研究表明，氧气输送是反应器放大的基本设计约束，因此，反应器性能的主要措施将是组织呼吸和生物量积累，分别通过废气分析和营养消耗相关性进行评估。 实验将阐明反应器性能的气体分散，液体混合和扩散的根组织内的氧气的作用。 气体分散将通过氩示踪分析/质谱法进行测量，而液体混合将通过浸没式和滴流床反应器操作的染料示踪剂进行评估。 此外，根轴内的氧扩散已被证明限制呼吸，因此，将进行一系列的根物种的根生长和呼吸的研究，以实验验证扩散控制模型的根生物需氧量。 ***

9522033 Curtis This proposal is on research to develop reactor design and operational principles for large-scale cultivation of root cultures for the production of biologically active chemicals such as pharmaceuticals, pesticides, and flavors. Preliminary studies have indicated that oxygen delivery is the fundamental design constraint for reactor scale-up.Therefore, the primary measures of reactor performance will be tissue respiration and biomass accumulation which are assessed by off-gas analysis and nutrient depletion correlations, respectively. Experiments will elucidate the role of gas dispersion, liquid mixing and diffusion of oxygen within the root tissue on reactor performance. Gas dispersion will be measured by argon tracer analysis/mass spectrometry, while liquid mixing will be assessed by dye tracers for both submerged and trickle-bed reactor operations. Also, oxygen diffusion within the root axis has been shown to limit respiration; therefore a study of root growth and respiration for a range of root species will be performed to experimentally validate a diffusionally controlled model for root biological oxygen demand. ***