SBIR Phase II: Genetic Fingerprinting and Registry for Psittacine Birds

*9529743巴顿这个小型企业创新研究第二阶段项目将为商业应用开发一个原型自动微卫星基因分型系统。在本项目的第一阶段，从风信子金刚鹦鹉中分离到了9个微卫星座位。这些基因座被用于风信子金刚鹦鹉个体和家系的DNA指纹分析。在其他六种金刚鹦鹉身上也发现了这些基因座的作用。在该项目的第二阶段，该系统将扩大到包括其他主要鹦鹉种群的六种鹦鹉：鹦鹉、东半球鹦鹉和东半球鹦鹉。利用在第一阶段开发的新的分子克隆方法，将分离和鉴定这六个新物种中每个物种的6-10个微卫星座位。将开发多重聚合酶链式反应(商标)反应，并在自动DNA测序仪上对电泳胶的单一通道中的多个基因座进行分析。基因分型数据将直接从实验室系统连接到计算机化的数据库。预计在第二阶段结束时，将有一个完整、有效和具有成本效益的系统来产生适合于大多数鹦鹉品种的个体特征和系谱分析的多位点基因。鹦鹉和包括牲畜、宠物、实验室动物和野生种群在内的许多其他物种的基因分型服务有很大的现有市场。随着《野生鸟类保护法》的实施和合法拥有的鸟类的身份确认，鹦鹉的这个市场预计将大幅增长。随着现代的、具有成本效益的系统的出现，例如本研究中将要开发的系统，预计其他物种的基因分型市场将迅速增长。***

*** 9529743 Patton This Small Business Innovation Research Phase II project will develop a prototype automated microsatellite genotyping system for commercial applications. In Phase I of this project, nine microsatellite loci were isolated from hyacinth macaws. These loci were used for DNA fingerprinting of individual hyacinth macaws and pedigrees. These loci were also found to work in six other macaw species. In Phase II of this project, the system will be expanded to include six species from the other major groups of psittacine birds: cockatoos, Old World Parrots, and New World Parrots. Using new molecular cloning methods, developed in Phase I, 6-10 microsatellite loci in each of the six new species will isolated and characterized. Protocols will be developed for multiplex PCR(TM) reactions and analysis of multiple loci in a single lane of an electrophoretic gel on an automated DNA sequencer. Genotype data will be linked directly from the laboratory system to a computerized database. It is anticipated that at the end of Phase II, a complete, efficient, and cost-effective system will be available for producing a multilocus genotype suitable for individual identity and pedigree analysis for most species of psittacines. There is a large existing market for genotyping services in parrots and many other species including livestock, pets, laboratory animals, and wild populations. This market is expected to grow substantially for parrots as the Wild Bird Conservation Act is enforced and positive identity of legally-owned birds is required. As modern, cost-effective systems, such as that to be developed in this research, become available it is anticipated that the market for genotyping other species will grow rapidly. ***