Chemical Monitoring Using Capillary Separations

这项工作，由助理教授罗伯特T。肯尼迪总统 佛罗里达大学以定性和定量问题为中心 与神经传递有关的化学物质的定量测量 中枢神经系统的神经系统。 支持由 化学系分析与表面化学专业。 结合毛细管分离技术的小型化取样系统 将以体内高速的长期目标开发 测量. 微透析和推拉灌注都将是 作为采样系统进行研究。 在线化学衍生将 产生适合于激光诱导荧光检测的产物，或 电化学检测 后者利用双缩脲反应形成 Cu（II）与肽类神经递质的络合物。 为了提高灵敏度， 将使用双电极检测器装置，其中复合物 在阳极和阴极之间来回摆动。 了解神经系统功能的化学基础 系统将在治疗神经系统疾病方面有很大的好处。 疾病 为了实现这一目标，必须确定和量化 化学品参与，并能够跟踪他们的变化暂时， 在空间上。 该项目的主要目标是提供解决方案 由于体积小，测量问题， 浓度，大量不同化学物质的存在， 在这种调查中遇到的短响应时间。

This work, directed by Assistant Professor Robert T. Kennedy of the University of Florida, is centered on problems of qualitative and quantitative measurement of chemicals associated with neurotransmission processes in the central nervous system. Support is provided by the Analytical and Surface Chemistry Program in the Chemistry Division. Miniaturized sampling systems coupled to capillary separation techniques will be developed with the long range goal of high speed in vivo measurements. Both microdialysis and push-pull perfusion will be investigated as sampling systems. On-line chemical derivatization will yield products suitable for laser induced fluorescence detection or electrochemical detection. The latter uses the biuret reaction to form Cu(II) complexes with peptide neurotransmitters. To enhance sensitivity, a dual electrode detector arrangment will be used in which the complex recycles back and forth between the anode and cathode. An understanding of the chemical basis of the functioning of the nervous system would be of great benefit in the treatment of neurological diseases. To achieve this, it is necessary to identify and quantify the chemicals involved and to be able to follow their changes temporily and spatially. The major objective of this project is to provide solutions to the measurement problems presented by the small volumes, the low concentrations, the presence of a large number of different chemicals and the short response time that are encountered in such investigations.