Integrating Laser Spectroscopic Studies in Biophysical Chemistry into Advanced Undergraduate Laboratory Courses

该系正在研制一种时间分辨荧光分光光度计，用于高级本科实验课程。该项目的主要活动是：(1)通过引入激光器来提高先进本科实验室的光谱成分并使其现代化；(2)利用荧光光谱作为工具，让学生了解当前生物物理化学的研究兴趣；(3)收集资料，强化课程中介绍的化学原理；(4)为学生提供物理化学表征和生物分子动力学研究的技术培训。所选的一组实验使用时间分辨激光荧光光谱技术进行数据收集，并使学生接触到抗体、核酸和血红素蛋白的研究，以及生物分子中发生的动态过程。实验还介绍了荧光光谱学的基本原理，并使学生接触到脉冲激光光谱学的现代技术。

The department is constructing a time-resolved fluorescence spectrophotometer for use in advanced undergraduate laboratory courses. The main activities of the project are (1) enhancing and modernizing the spectroscopy component in advanced undergraduate laboratories by the introduction of lasers; (2) using fluorescence spectroscopy as a tool to expose students to current research interests in biophysical chemistry; (3) collecting data to reinforce chemical principles introduced in the courses; and (4) providing students with training in techniques for both the physical-chemical characterization and dynamical study of biomolecules. The chosen set of experiments uses time-resolved laser fluorescence spectroscopic techniques for data collection and exposes students to research on antibodies, nucleic acids, and heme proteins, and to the dynamical processes occurring in biomolecules. The experiments also introduce the fundamentals of fluorescence spectroscopy as well as exposing students to modern techniques in pulsed laser spectroscopy.