Modular Approach to Biotechnology Laboratory Instruction Based on a Novel Green-Fluorescent Protein

基于绿色荧光蛋白的生物技术实验模块化教学 该项目正在为中学和大学生及其教师开发生物技术实验室模块，其基础是一种独特的蛋白质，绿色荧光蛋白（GFP），该蛋白质可作为基因表达和蛋白质纯化所有步骤的直观报告者。检测只需要一个长波紫外线灯，使该系统适用于低预算的课程。由于蛋白质是如此容易可视化，它提高了学生的分子生物学和生物化学的理解，使实验室练习的每一步都是一个令人兴奋的经历。基于GFP的实验室模块的最初概念于1989年在罗格斯大学开发，并已成功地进行了五年的“实地测试”。其他九个机构，包括一个其他主要的研究型大学，一个四年制学院，两个县学院，三所高中，一个主要的生化供应公司，和一个国家实验室加入罗格斯大学在一个非正式的财团，以推进基于绿色荧光蛋白的实验室模块的概念。最初是从一种生物发光水母中分离出来的。GFP现在可以克隆到其他生物体中。因此。该基因及其蛋白可广泛使用。该项目正在开发以绿色荧光蛋白为基础的实验室模块，适合高中和大专水平。模块化“试剂盒”的方法来教生物技术技术允许蛋白质纯化和重组DNA技术转移到所有适当的设置只有很小的修改。这种基于绿色荧光蛋白的模块化方法对技术人员的培训方式带来了重大变化，可以在全国范围内广泛应用于生物技术界。

Modular Approach to Biotechnology Laboratory Instruction Based on a Novel Green-Fluorescent Protein This project is developing laboratory modules in biotechnology for secondary school and undergraduate level students and their teachers, based on a unique protein, the green-fluorescent protein (GFP), which serves as an easily visualized reporter for gene expression and all steps in protein purification. Detection requires only a long-wave UV lamp, making the system adaptable to low-budget curricula. Because the protein is so easily visualized, it enhances the students' understanding of molecular biology and biochemistry making each step in the laboratory exercise an exciting experience. Initial concepts for GFP-based laboratory modules were developed at Rutgers in 1989 and have been successfully "field tested" for five years. Nine other institutions including one other major research university, one four-year college, two county colleges, three high schools, one major biochemical supply corporation, and one national laboratory are joining Rutgers in an informal consortium to advance the GFP-based laboratory module concept. Originally isolated from a bioluminescent jellyfish. GFP can now be cloned into other organisms. Thus. the gene and its protein are available for widespread use. The project is developing GFP-based laboratory modules geared for the advanced secondary school and junior college levels. The modular "kit" approach to teaching biotechnology techniques allows both protein purification and recombinant DNA techniques to be transferred to all appropriate settings with only minor modifications. This GFP-based modular approach provides major changes in the way technicians are trained and can be projected on a national scale to be widely applied in the biotechnology community.