Acqisition of Biophysical Instrumentation for Molecular Recognition Studies

本申请要求获得用于分子识别研究的生物物理仪器的资金。该仪器为等温滴定量热计（MicroCal）。等温滴定量热法直接测量蛋白质-配体相互作用的热力学。目前正在研究的系统有：铁氧还蛋白连接的叶绿体酶中的蛋白质-蛋白质相互作用；与cAMP和双链DNA相互作用的cAMP受体蛋白（CRP）；冷休克蛋白与特定单链DNA序列相互作用；甾醇及其衍生物与(S)-腺苷- l-蛋氨酸的相互作用；24(25)-甾醇甲基转移酶；金属内酰胺酶与内酰胺类抗生素的相互作用。直接确定这些系统中的结合热力学，表征结合时的构象变化，以及对它们的分析将补充和扩展以前的生化结果。

This application requests funds for the acquisition of biophysical instrumentation for molecular recognition studies. The instrument is an isothermal titration calorimeter (MicroCal). Isothermal titration calorimetry directly measures the thermodynamics of protein-ligand interaction. The systems currently under study are: protein- protein interactions in ferredoxin-linked chloroplast enzymes; cAMP receptor protein (CRP) interacting with cAMP and double stranded DNA; cold shock protein interacting with the specific single-stranded DNA sequences; interactions of sterol and sterol derivatives with (S)-adenosyl-L- methionine;24(25)-sterol methyl transferase (SMT); interactions of metallo--lactamases with -lactam antibiotics. Direct determination of the thermodynamics of binding in these systems, characterization of conformational changes upon binding, and their analysis will complement and extend previous biochemical results.