An Optical Biosensor Instrument for Analysis of Biomolecular Interactions in Molecular and Cellular Biology

这是一份购买用于分子和细胞生物学研究的光学生物传感器仪器的提案。建议的仪器使用基于试管的设计来实时测量不同分子之间的相互作用。这项技术是基于一种光学生物传感器，它检测由于生物传感器表面边界折射率变化而产生的大分子结合。这种仪器可以对分子相互作用进行实时分析。我们将研究各种分子间的相互作用。这些包括激素-受体、酶-底物、受体-细胞、DNA-DNA、DNA-蛋白质和蛋白质-蛋白质相互作用的动力学和热力学分析。这些相互作用是分子和细胞生物学过程的核心，如细胞运动、基因重组、蛋白质运输、RNA转录和信号转导。现在可以在大小和浓度的广泛动态范围内实时研究这些相互作用，而不需要放射性同位素或其他标记。这项新技术的应用将极大地促进细胞功能的基础研究，并将有助于培养未来的生物科学家。

This is a proposal to purchase an optical biosensor instrument for research in molecular and cellular biology. The proposed instrument uses a cuvette-based design to measure, in real-time, the interaction between different molecules. The technology is based on an optical biosensor which detects macromolecular binding due to changes in refractive index at the boundary of the biosensor surface. This instrument permits real-time, analysis of molecular interactions. A variety of molecular interactions will be studied. These include both kinetic and thermodynamic analyses of hormone-receptor, enzyme-substrate, receptor- cell, DNA-DNA, DNA-protein, and protein-protein interactions. These interactions are central to processes in molecular and cellular biology such as cell motility, genetic recombination, protein transport, RNA transcription, and signal transduction. These interactions can now be studied in real time over a wide dynamic range of size and concentration, and without the need for radioisotopic, or other, labeling. Application of this new technology will greatly facilitate fundamental research on cellular function and will contribute to the training of future biological scientists.