Japan JSPS Program: First Feeding and Nutrient Uptake by Paralavae of the Squid Todarodes Pacificus

鲍尔9605828 该奖项支持了为期24个月的日本科学促进协会（JSPS）博士后奖学金博士约翰鲍尔在北海道大学，函馆，日本。 Bower博士将与渔业学院副教授Yasunori Sakurai博士合作开展一项名为“太平洋鱿鱼的第一次摄食和营养吸收”的研究项目。" 许多软体海洋无脊椎动物的幼虫显然能够直接从海水中吸收溶解的有机物质以满足营养需求。 这项拟议中的研究将建立在现有的研究基础上，并检查Ommastrephidae家族的幼鱿鱼是否可以从海水中运输氨基酸。 通过人工受精饲养的幼鱼将暴露于海水介质中的放射性标记底物，以确定海水介质中的放射性是否增加。 为了研究流入量和净通量之间的关系，将通过高效液相色谱法测定氨基酸从溶液中的去除速率来测量氨基酸的摄取。 此外，还将检查野外捕获的幼龟的胃，以确定早期的猎物。 目前，人工饲养小眼蝇是研究生命早期阶段的唯一方法。 本研究的另一个目的是发展一种方法来饲养小眼鲷科孵化扩大实验室研究。 如果成功的饲养方法被开发出来，那么这个具有重要商业价值的家庭的早期生活阶段的具体方面就可以确定了。 ***

Bower 9605828 This award supports a 24 month Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Dr. John Bower at Hokkaido University, Hakodate, Japan. Dr. Bower will work with Dr. Yasunori Sakurai, Associate Professor in the Faculty of Fisheries, on a research project entitled, "First Feeding and Nutrient Uptake by Para-Larvae of the Squid Todarodes Pacificus." The larvae of many soft-bodied marine invertebrates are apparently capable of absorbing dissolved organic materials directly from sea water to meet nutritional requirements. The proposed research will build upon existing research and examine if hatchling squids of the family Ommastrephidae can transport amino acids from sea water. Hatchlings reared through artificial fertilization will be exposed to radio- labeled substrates in a sea water medium to determine if an increase in radioactivity in the sea water medium occurs. To investigate the relationship between influx and net flux, the uptake of amino acids will be measured by determining the rate of removal of amino acids from solution by high- performance liquid chromatography. In addition, the stomachs of field-caught hatchlings will be examined to identify early prey. At present, rearing ommastrephidae young in captivity is the only way to study early stages of life. A further goal of this research is to develop a method to rear ommastrephidae hatchlings for extended laboratory studies. If successful rearing methods are developed, specific aspects of the early life stages of this commercially important family can then be ascertained. ***