Electroactive Oligodeoxynucleotides

该项目由分析和表面化学项目支持，重点研究在电极表面固定时具有特定和稳定电化学信号的寡脱氧核苷酸的合成。田纳西大学的James Q. Chambers教授和他的学生将通过磷酰胺连接剂对醌进行汞化和偶联，使这些寡脱氧核苷酸具有电活性。这些固定试剂在暴露于具有互补碱基序列的DNA分子时将表现出电化学反应的变化，从而为特定DNA分子提供高选择性和敏感的生物传感器。电化学、光谱电化学、石英晶体微天平和阻抗光谱测量将用于表征这些生物传感器。田纳西大学的钱伯斯教授和他的学生将在这项工作中构建和表征能够选择性和敏感地检测目标DNA分子的生物传感器。寡脱氧核苷酸将被修饰成具有电活性，这样当它们与电极表面的目标DNA复合时，就可以检测到电分析电流。这些生物传感器在分析具有特定碱基序列的多核苷酸方面具有广泛的应用潜力。

This project, supported in the Analytical and Surface Chemistry Program, focusses on the synthesis of oligodeoxynucleotides that have specific and stable electrochemical signals when immobilized on an electrode surface. Professor James Q. Chambers and his students at the University of Tennessee will use mercuration and coupling of quinones via phosphoramidite linking agents to impart electroactivity to these oligodeoxynucleotides. These immobilized reagents will exhibit shifts in their electrochemical responses upon exposure to DNA molecules with complementary base sequences making a highly selective and sensitive biosensor for specific DNA molecules. Electrochemical, spectroelectrochemical, quartz crystal microbalance, and impedance spectroscopic measurements will be used to characterize these biosensors. Biosensors that can selectively and sensitively detect target DNA molecules will be constructed and characterized in this work by Professor Chambers and his students at the University of Tennessee. Oligodeoxynucleotides will be modified to become electroactive so that when they complex with target DNA on the surface of an electrode, an electroanalytical current can be detected. These biosensors have extensive potential for application in the analysis of polynucleotides with specific base sequences.