Analysis of DNA From Permafrost Specimens

本研究提出利用新的分子方法，从阿拉斯加和育空地区的永久冻土遗骸密集沉积物中恢复，扩增和测序DNA，这些沉积物代表了过去40,000年来该地区动物居民的独特样本。pi提出了一种小型的、创新的和实验性的方法，这种方法有很高的可能性获得数据，可以解决极地生物学中长期存在的问题，包括人口流动、灭绝和生物适应。基础研究技术包括使用古代DNA技术提取和表征永久冻土稳定标本中保存的DNA。

This study proposes to utilize new molecular approaches to recover, amplify, and sequence DNA from dense deposits of permafrost remains in Alaska and the Yukon which represent unique samples of the faunal inhabitants of the area over the past 40,000 years. The PIs propose a small, innovate and experimental approach which has a high possibility of obtaining data that can address long standing questions in polar biology including population movements, extinctions, and biotic adaptations. The basic research techniques involve using ancient DNA techniques to extract and characterize preserved DNA in permafrost stabilized specimens.