Interactions Between Bacterioplankton Communities and Dissolved Substrates at the Bermuda Atlantic Time-Series Study Station

用浮游细菌核糖体rna的系统发育类群特异性探针进行的杂交测量表明，在马尾藻海西北部的较低海洋表层，几个主要的浮游细菌类群形成了高度分层的种群。在同一地点进行的其他研究发现了季节性循环的证据，其中溶解的有机碳（DOC）在春季期间在上层表层积累，并在冬季混合期间出口到下层表层。本研究拟通过不同深度的水与接种剂相结合的海水培养实验，研究浮游细菌群落组成与水体DOC来源、数量和不稳定性的关系。扩增核糖体RNA基因的高分辨率电泳图将用于调查群落组成。电泳结果将通过将总群落RNA与系统发育群体特异性探针杂交来证实。这项研究的数据将与正在进行的浮游细菌产量、生物量和DOC的测量数据进行比较，这些数据是百慕大大西洋时间序列研究的一部分。

Hybridization measurements made with phylogenetic group-specific probes for bacterioplankton ribosomal RNAs have shown that several major bacterioplankton groups form highly stratified populations in the lower ocean surface layer in the northwestern Sargasso Sea. Other studies at the same site have uncovered evidence of a seasonal cycle in which dissolved organic carbon (DOC) accumulates in the upper surface layer during the vernal period and is exported to the lower surface layer during winter mixing. This study proposes to examine the relationship between the composition of bacterioplankton communities and the source, amount and lability of DOC in the water column by conducting seawater culture experiments that combine water and inocula from different depths. High-resolution electropherograms of amplified ribosomal RNA genes will be used to survey community composition. Electropherogram results will be confirmed by hybridizations of total community RNA to phylogenetic group-specific probes. Data from this study will be compared to ongoing measurements of bacterioplankton production, biomass and DOC that are a part of the Bermuda Atlantic Time-Series Study.