Engineering Research Equipment: Tissue and Cellular Engineering Laboratory

9622411摩尔该提案支持购买组织和细胞工程设备，这些设备将专门用于支持佛罗里达国际大学的工程研究。该设备将用于几个研究项目，特别是一项题为“流动和拉伸对血管内皮细胞的影响”的研究。“该项目旨在定量确定动脉中的机械环境如何影响动脉粥样硬化的形成。排列在动脉内表面的细胞受到血液流动的剪切应力和心脏收缩期间动脉径向扩张产生的周向应力。以前的研究集中在剪切应力或径向膨胀，但不是两者兼而有之。在这项研究中，细胞将被培养在管道的内表面上，管道的机械行为类似于动脉，然后受到现实的机械条件，包括剪应力和径向膨胀。将使用现代生物技术测量被认为在动脉粥样硬化形成中重要的物质的分泌。由主要研究者指导的其他项目将受益于该设备，包括冠状动脉中的流动研究和血管支架的流动分析。在PI的部门内，至少有五个其他项目将能够使用拟议的设备。***

9622411 Moore This proposal supports the purchase of tissue and cellular engineering equipment which will be dedicated to support research in engineering at Florida International University. The equipment will be used for several research projects, including in particular a study entitled "Flow and Stretch Effects on Vascular Endothelial Cells." This project aims to quantitatively identify how the mechanical environment in arteries affects the formation of atherosclerosis. The cells that line the inner surfaces of arteries are subjected to both shear stress from the flow of blood, and the circumferential stress that results from the radial expansion of the artery during heart contraction. Previous research has concentrated on either shear stress or radial expansion, but not both. In this research, cells will be cultured onto the inner surfaces of tubes which behave mechanically like arteries, then subjected to realistic mechanical conditions including both shear stress and radial expansion. The secretion of substances thought to be important in the formation of atherosclerosis will be measured using modern biological techniques. Other projects directed by the principal investigator that will benefit from this equipment include flow studies in the coronary arteries and flow analysis of vascular stents. Within the PI's department, there are at least five other projects that will be able to make use of the proposed equipment. ***