Gas-Phase Proteins: Conformer Separation and Identification, Folding Dynamics and Stability

这是一个教师早期职业项目。该项目将研究气相蛋白质的折叠和结构。蛋白质的三级/四级结构对其活性至关重要。气相蛋白质是无溶剂的，结构上仅由其分子内约束来定义。因此，气相工作为理解溶液中的折叠和结构提供了背景，本项目将阐明分子内和溶剂相互作用在建立溶液中蛋白质结构中的作用。这项工作将使用离子色谱法，一种分离气相异构体的新技术，结合电喷雾质谱法。这项技术将提供所研究蛋白质的整体形状信息。在教育方面，克莱默博士将通过开发学生经营的生物分析化学系列研讨会来教授研究生口头沟通技巧，并将定期指导少数民族学生。该项目将研究最简单的情况下的蛋白质结构，即气相，并将利用由此获得的信息来了解控制其溶液结构的因素。该项目将教授研究生有效的口头交流，克莱默博士将在他的研究小组中定期指导少数民族学生。

This is a Faculty Early Career project. The project will study gas phase protein folding and structure. The solution tertiary/quaternary structure of proteins is crucial to their activity. Gas phase proteins are solvent-free, and are structurally defined only by their intramolecular constraints. Gas phase work thus provides a background for understanding folding and structure in solution, and the present project will elucidate the roles that intramolecular and solvent interactions play in establishing protein structure in solution. This work will use ion chromatography, a new technique for separating isomers in the gas phase, combined with electrospray mass spectrometry. The technique will provide overall shape information about the proteins studied. Educationally, Dr. Clemmer will teach oral communication skills to graduate students by development of a student-run bioanalytical chemistry seminar series and will mentor minority students regularly. This project will study protein structure in the simplest of situations, the gas phase, and will use the information gained thereby to understand the factors governing their solution structures. The project will teach effective oral communication to graduate students and Dr. Clemmer will mentor minority students regularly in his research group.