CAREER: Site Selective Radical Induced Dissociation of Whole Proteins in the Gas Phase

职业：气相中全蛋白质的位点选择性自由基诱导解离

• 批准号: 0747481
• 负责人: Ryan Julian
• 金额: $ 57.5万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0747481&HistoricalAwards=false
• 关键词: CAREER; Site; Selective; Radical; Induced

This CAREER award by the Analytical and Surface Chemistry program supports work by Professor Ryan Julian at the University of California - Riverside to develop faster methods for proteomics - the identification of proteins in a given cell or environment. Proteins are the biological agents which carry out virtually all chemistry in living organisms. Identifying all proteins present is often one of the first steps towards obtaining a detailed understanding of a biochemical process. Rapid identification is difficult because there are often thousands of proteins involved in complex functional relationships in a given site. Professor Julian and his group utilize a novel combination of mass spectrometry and laser induced photodissociation to facilitate identification. The information obtained from these experiments can uniquely identify a protein in a fraction of a second. This research simultaneously serves many educational purposes, including the training of graduate, postgraduate, and undergraduate researchers in the important and growing field of biological mass spectrometry. Related outreach activities catering to the specific needs of the diverse student body at UC-Riverside include a program for the formal mentoring of science undergraduates on campus with an emphasis on helping underrepresented groups and student-athletes. A new graduate course is being created to enhance student knowledge of chemical and noncovalent bonds and to emphasize the importance of connections between all fields of chemistry.

该职业奖由分析和表面化学项目颁发，支持加州大学河滨分校 Ryan Julian 教授的工作，以开发更快的蛋白质组学方法，即在给定细胞或环境中识别蛋白质。 蛋白质是生物体中几乎进行所有化学反应的生物制剂。 识别所有存在的蛋白质通常是详细了解生化过程的第一步。 快速识别很困难，因为在给定位点通常有数千种蛋白质参与复杂的功能关系。朱利安教授和他的团队利用质谱法和激光诱导光解离的新颖组合来促进识别。 从这些实验中获得的信息可以在几分之一秒内唯一地识别蛋白质。 这项研究同时服务于许多教育目的，包括在重要且不断发展的生物质谱领域培训研究生、研究生和本科生研究人员。满足加州大学河滨分校不同学生群体的具体需求的相关外展活动包括对校园理科本科生进行正式指导的计划，重点是帮助代表性不足的群体和学生运动员。 正在创建一门新的研究生课程，以增强学生对化学和非共价键的知识，并强调化学所有领域之间联系的重要性。