CAREER: Site Selective Radical Induced Dissociation of Whole Proteins in the Gas Phase

该职业奖由分析和表面化学项目颁发，支持加州大学河滨分校的Ryan Julian教授开发更快的蛋白质组学方法，即在给定细胞或环境中识别蛋白质。蛋白质是生物制剂，它几乎完成了生物体中所有的化学反应。识别所有存在的蛋白质通常是获得详细了解生化过程的第一步。快速鉴定是困难的，因为在一个给定的位点上通常有数千种蛋白质参与复杂的功能关系。朱利安教授和他的团队利用质谱法和激光诱导光解的新组合来促进鉴定。从这些实验中获得的信息可以在几分之一秒内唯一地识别蛋白质。这项研究同时服务于许多教育目的，包括培养重要和不断发展的生物质谱领域的研究生、研究生和本科生研究人员。为满足加州大学河滨分校不同学生群体的具体需求，该校开展了相关的拓展活动，其中包括对在校理科本科生进行正式指导的项目，重点是帮助代表性不足的群体和学生运动员。正在开设一门新的研究生课程，以提高学生对化学键和非共价键的认识，并强调化学各领域之间联系的重要性。

This CAREER award by the Analytical and Surface Chemistry program supports work by Professor Ryan Julian at the University of California - Riverside to develop faster methods for proteomics - the identification of proteins in a given cell or environment. Proteins are the biological agents which carry out virtually all chemistry in living organisms. Identifying all proteins present is often one of the first steps towards obtaining a detailed understanding of a biochemical process. Rapid identification is difficult because there are often thousands of proteins involved in complex functional relationships in a given site. Professor Julian and his group utilize a novel combination of mass spectrometry and laser induced photodissociation to facilitate identification. The information obtained from these experiments can uniquely identify a protein in a fraction of a second. This research simultaneously serves many educational purposes, including the training of graduate, postgraduate, and undergraduate researchers in the important and growing field of biological mass spectrometry. Related outreach activities catering to the specific needs of the diverse student body at UC-Riverside include a program for the formal mentoring of science undergraduates on campus with an emphasis on helping underrepresented groups and student-athletes. A new graduate course is being created to enhance student knowledge of chemical and noncovalent bonds and to emphasize the importance of connections between all fields of chemistry.