CAREER: Site Selective Radical Induced Dissociation of Whole Proteins in the Gas Phase

这项由分析和表面化学项目颁发的职业奖支持加州大学河滨分校的Ryan Julian教授开发更快的蛋白质组学方法-在给定的细胞或环境中识别蛋白质。蛋白质是一种生物制剂，几乎完成了生物体中所有的化学作用。确定所有存在的蛋白质通常是获得对生化过程的详细了解的第一步之一。快速鉴定是困难的，因为在一个给定的位置，通常有数千个蛋白质参与复杂的功能关系。朱利安教授和他的团队利用一种新的质谱学和激光诱导光解离的组合来促进鉴定。从这些实验中获得的信息可以在不到一秒的时间内唯一地识别蛋白质。这项研究同时服务于许多教育目的，包括在重要的和不断增长的生物质谱学领域培训研究生、研究生和本科生研究人员。针对加州大学河滨分校不同学生群体的具体需求，相关的外展活动包括在校园内为理科本科生提供正式指导的计划，重点是帮助代表性不足的群体和学生运动员。正在开设一门新的研究生课程，以加强学生对化学和非共价键的知识，并强调化学所有领域之间联系的重要性。

This CAREER award by the Analytical and Surface Chemistry program supports work by Professor Ryan Julian at the University of California - Riverside to develop faster methods for proteomics - the identification of proteins in a given cell or environment. Proteins are the biological agents which carry out virtually all chemistry in living organisms. Identifying all proteins present is often one of the first steps towards obtaining a detailed understanding of a biochemical process. Rapid identification is difficult because there are often thousands of proteins involved in complex functional relationships in a given site. Professor Julian and his group utilize a novel combination of mass spectrometry and laser induced photodissociation to facilitate identification. The information obtained from these experiments can uniquely identify a protein in a fraction of a second. This research simultaneously serves many educational purposes, including the training of graduate, postgraduate, and undergraduate researchers in the important and growing field of biological mass spectrometry. Related outreach activities catering to the specific needs of the diverse student body at UC-Riverside include a program for the formal mentoring of science undergraduates on campus with an emphasis on helping underrepresented groups and student-athletes. A new graduate course is being created to enhance student knowledge of chemical and noncovalent bonds and to emphasize the importance of connections between all fields of chemistry.