Capillary Electrophoresis in the Analytical and Physical Chemistry Laboratories

自上世纪80年代初S提出以来，毛细管电泳法已发展成为生物大分子高分辨率分离的重要工具。不断开发的方法已经将应用扩展到各种大小的带电和中性化合物。为了为未来的就业和升学做准备，学生们应该拥有比教科书更多的关于这种工具方法的知识。为了提供这种经验，正在为三个高级化学实验室开发涉及使用CE系统的实验。仪器分析专业的学生正在使用反相液相色谱和毛细管电泳法分析一种商品感冒/鼻窦药物。他们正在观察两种分离方法的差异，以及洗脱液性质和pH的重要性。在标准的物理化学实验室里，学生们正在测量几个基本的物理参数，这些参数是电泳法的基础。一个新的物理化学实验室，强调生物和合成大分子的性质，将作为标准课程的替代课程提供。选择该选项的学生使用CE来跟踪酶催化反应的动力学。

Since its introduction in the early 1980's, capillary electrophoresis (CE) has grown to become an essential tool for high resolution separations of biological macromolecules. Continuing method development has extended applications to a wide variety of charged and neutral compounds of all sizes. To be prepared for future employment and advanced schooling, students should have more than textbook knowledge of this instrumental method. To provide this experience, experiments that involve use of a CE system are being developed for three upper-level chemistry laboratories. Students in instrumental analysis are analyzing a commercial cold/sinus remedy using both reversed-phase liquid chromatography and capillary electrophoresis. They are observing the differences in the two separation methods and the importance of eluent character and pH. Students in the standard physical chemistry laboratory are measuring several physical parameters fundamental to the electrophoresis process. A new physical chemistry laboratory, emphasizing the properties of biological and synthetic macromolecules is being offered as an alternate to the standard course. Students who choose that option use CE to follow the kinetics of an enzyme-catalyzed reaction.