Structural biology of plant potyvirus and cytomegalovirus effector proteins (A03)

植物马铃薯Y病毒和后生巨细胞病毒(CMV)感染机制的结构决定因素将在A3中描述。由CMV蛋白IE1和pUL50-pUL53以及植物马铃薯Y病毒衣壳蛋白形成的高级寡聚复合体将被产生和结晶。这些结晶学研究将通过对IE1和pUL50-pUL53的现有晶体进行X射线结晶学工作的碎片筛选、交联实验和诱变调查以及我们项目A2、A7、B3、C2和C3的深入研究来平行进行。

The structural determinants of plant potyvirus and metazoan cytomegalovirus (CMV) infection mechanisms will be characterized in A3. Higher oligomeric complexes formed by the CMV proteins IE1 and pUL50-pUL53 as well as plant potyvirus capsid proteins will be produced and crystallized. These crystallographic studies will be paralleled by fragment screening by X-ray crystallography efforts with available crystals of IE1 and pUL50-pUL53, crosslinking experiments and mutagenesis inquiries as well as in-depth studies by our collaborators from projects A2, A7, B3, C2 and C3.