Cell wall properties and bioadhesion in different genotypes of the aeroterrestrial green algal genus Klebsormidium from alpine soils of the Austrian Alps

陆生绿色微藻是阿尔卑斯高寒地区生物土壤结皮的典型组成部分。在这里，它们与土壤颗粒形成最上层毫米的密切联系，从而在初级生产、养分循环、保水和土壤稳定中发挥重要的生态作用。由于对这些生物的干燥胁迫和耐受策略的研究通常很少，因此将从600 - 3000m的高山土壤中分离出具有代表性的Klebsormidium属藻类菌株，对其潜在的细胞学和生态生理机制进行全面而精确的评估。由于海拔的差异，生活在高山地区的生物必须很好地适应包括干旱在内的剧烈的非生物梯度，以保证长期生存。在克雷伯sordium分离株中研究的机制包括它们的生理（如生长耐受性模式）和在一组受控的实验室暴露条件下的超微结构，使用各种细胞生物学标记（如细胞器和膜完整性，细胞骨架可视化）以及生长和光合作用在补液后恢复的能力。主要目的是评估藻细胞的结构完整性/稳定性是否有助于从干燥胁迫中快速恢复。

Aeroterrestrial green microalgae are typical components of biological soil crusts in alpine regions of the Alps. Here they form in the uppermost millimetres an intimate association with soil particles, thereby exerting important ecological roles in primary production, nutrient cycling, water retention and stabilization of soils. Since desiccation stress and tolerance strategies of these organisms are generally badly studied, a comprehensive and precise evaluation of the underlying cytological and ecophysiological mechanisms will be carried out with representative algal strains of the genus Klebsormidium which were isolated from alpine soils between 600 and 3000m. Because of altitudinal differences organisms living in alpine regions have to be well adapted to dramatic abiotic gradients including drought to guarantee long-term survival. The mechanisms to be studied in the Klebsormidium isolates include their physiology (e.g. growth tolerance patterns) and ultrastructure under a set of controlled laboratory exposure conditions using various cell biological markers (e.g. organelles and membrane integrity, cytoskeleton visualisation) and the capability of growth and photosynthesis to recover after rehydration. The main goal is to evaluate whether the structural integrity/stability of the algal cell may contribute to a quick recovery from desiccation stress.