Glycosylation of Recombinant Proteins Research Planning Grant

9711130 Harcum这项研究计划资助提案涉及了一个似乎具有商业意义和科学研究不足的领域，即生物反应器条件对蛋白质糖基化模式的影响。 该研究计划将研究哺乳动物细胞生长在连续培养（chemosts），并确定变量的影响，如搅拌，pH值，温度，生长速率等，电泳和色谱分离，质谱和NMR分析将用于结构测定。 ***

9711130 Harcum This Research Planning Grant proposal addresses what appears to be a commercially significant and scientifically under- investigated area, the effect of bioreactor conditions on the glycosylation patterns of protein. The research program would study mammalian cells grown in continuous culture (chemosts) and determine the effect of variables such as agitation, pH, temperature, growth rates, etc. Electrophoretic and chromatographic separations coupled with mass spec and NMR analyses will be used in structural determinations. ***