CAREER: Structural Studies of Transcription Regulation by RNA-Protein Interactions

9722392帕尔默描述了一项职业发展计划，该计划结合了核磁共振波谱对结构/功能的研究和教学。该研究计划将为理解RNA-蛋白质复合体中的分子识别以及Lambdoid大肠杆菌噬菌体中的转录终止/反终止提供分子基础。最初的目标是表征HK022 Nun蛋白和Box B RNA序列之间的相互作用。这项计划的教学目标是开发高分辨率核磁共振波谱教学和自学的资源。高分辨率核磁共振波谱是学术和工业环境中化学和生物研究的一个日益重要的组成部分，越来越多的大学开设了核磁共振波谱课程。教育工作是基于教科书，题为蛋白质核磁共振光谱学：原理和实践，由该提案的PI共同撰写。课程材料将在哥伦比亚大学教授的课程“生物大分子的核磁共振光谱学”中使用，并将通过互联网分发。了解蛋白质识别和与其他分子相互作用的机制是化学、生物物理学和生物技术中的一个重要研究问题。这项建议的研究部分将使用核磁共振光谱学来阐明蛋白质和RNA分子之间相互作用的性质，这对分子识别非常重要。教育部分将开发基于计算机的材料，用于在高等本科生和研究生水平上教授核磁共振光谱学。教材将补充由国际和平研究所合著的名为《蛋白质核磁共振波谱：原理与实践》的教科书。***

9722392 Palmer A career development program is described that integrates research and pedagogy of structure/function by NMR spectroscopy. The research program will provide a molecular basis for understanding molecular recognition in RNA-protein complexes as well as transcription termination/antitermination in lambdoid coliphages. The initial goal is the characterization of the interaction between HK022 Nun protein and Box B RNA sequences. The educational objectives of this proposal are the development of resources for the teaching and self-study of high resolution NMR spectroscopy. High-resolution NMR spectroscopy is an increasingly important component of chemical and biological research in academic and industrial settings, and courses in NMR spectroscopy are offered at an expanding number of universities. The educational efforts are based on the textbook, entitled Protein NMR Spectroscopy: Principles and Practice, co-authored by the PI of this proposal. Course materials will be used in the course "NMR spectroscopy of biological macromolecules", taught at Columbia University, and will be distributed via the Internet. Understanding the mechanisms by which proteins specifically recognize and interact with other molecules is a critical research problem in chemistry, biophysics, and biotechnology. The research component of this proposal will use NMR spectroscopy to elucidate the nature of the interactions between proteins and RNA molecules that are important for molecular recognition. The educational component will develop computer-based materials for teaching NMR spectroscopy at the advanced undergraduate and graduate university levels. The educational materials will complement the textbook, entitled Protein NMR Spectroscopy: Principles and Practice, co-authored by the PI. ***