A Novel Approach to the Crystallization of Macromolecules Using Self-Assembling Matrices and Phage Display

9723244 Momany本研究项目的总体目标是开发基于自组装矩阵（SAM）设计的新型结晶策略。 为了创建这些SAM，将使用复杂的分子技术和组合文库来工程化抗体片段和其他相关的大分子，这些大分子以可以容纳额外的靶大分子的大空隙结晶。 通过噬菌体展示平移选择的特定相互作用，将需要结构的靶标携带到网格中。 基于SAM的结晶方法将显着提高晶体结构测定的速率，从而使实际的晶体学与基因组计划相关联。 该方法是结晶大分子的理想选择，如肽激素、碳水化合物、RNA、DNA和膜结合或糖基化蛋白质，这些蛋白质在过去抵抗标准的基于结晶的结晶策略。 通过创建一种避免经验结晶筛选的机制，这种基于SAM的方法可以对整个结构生物学领域产生重大影响。 ***

9723244 Momany The overall goal of this research project is to develop novel crystallization strategies based on the design of self-assembling matrices (SAMs). To create these SAMs, sophisticated molecular techniques and combinatorial libraries will be used to engineer antibody fragments and other relevant macromolecules that crystallize with large voids that can accommodate additional target macromolecules. The target for which a structure is desired would be carried into the lattice though specific interactions selected though Phage Display panning. A SAM-based method of crystallization will significantly increase the rate of crystal structure determinations and thus make practical crystallography associated with genome-projects. The approach is ideal for crystallizing macromolecules such as peptide hormones, carbohydrates, RNA, DNA, and membrane-bound or glycosylated proteins that have resisted standard empirically-based crystallization strategies in the past. By creating a mechanism that avoids empirical crystallization screens, this SAM-based approach can have a significant impact on the entire field of structural biology. ***