Molecular and Genetic Analyses of the Carbon Concentrating Mechanism of Chlamydomonas reinhardtii

本研究的主要目的是阐明该藻类碳浓度调节机制的组成和机制。这项工作源于通过对高二氧化碳需求突变菌株的基因组互补检测CAH3基因。在此筛选之后，正在分离其他基因。c02响应启动子将用于构建一个基因盒，该基因盒允许在另一种方法中筛选可选择的标记，以定义从CO2传感到基因表达的信号转导链中的突变体。这些突变体将被描述。

9723333 Weeks The primary goal of the research is to elucidate components and mechanisms of regulation of the carbon concentration mechanism in this alga. The work originates from the detection of gene CAH3 through genomic complementation of a high CO2-requiring mutant strain. Additional genes are being isolated following this screen. A C02-responsive promoter will be used to construct a gene cassette that allows screening for a selectable marker in another approach to defining mutants in the signal transduction chain leading from CO2 sensing to gene expression. These mutants will be characterized.